Fermentative Enzymes Production Efficiency of Ethanologenic Bacteria Isolated from Raphia Palm Sap
Published: 2021-03-02
Page: 27-48
Issue: 2021 - Volume 4 [Issue 1]
O. A. Ayanshina
*
Department of Biochemistry, Faculty of Basic Medical Sciences, College of Medicine, University of Lagos, P.M.B 12003, Lagos State, Nigeria. and Department of Chemical Sciences, College of Natural and Applied Sciences, Crescent University, Abeokuta, Ayetoro Road, Lafenwa, P.M.B. 2104, Sapon, Abeokuta, Ogun State, Nigeria.
M. N. Igwo-Ezikpe
Department of Biochemistry, Faculty of Basic Medical Sciences, College of Medicine, University of Lagos, P.M.B 12003, Lagos State, Nigeria.
N. O. A. Imaga
Department of Biochemistry, Faculty of Basic Medical Sciences, College of Medicine, University of Lagos, P.M.B 12003, Lagos State, Nigeria.
T. O. Ajasa
Department of Biochemistry, Faculty of Basic Medical Sciences, College of Medicine, University of Lagos, P.M.B 12003, Lagos State, Nigeria.
O. Adeboye
Department of Biochemistry, Faculty of Basic Medical Sciences, College of Medicine, University of Lagos, P.M.B 12003, Lagos State, Nigeria.
I. A. Odjokpa
Department of Biochemistry, Faculty of Basic Medical Sciences, College of Medicine, University of Lagos, P.M.B 12003, Lagos State, Nigeria.
S. E. Agbale
Department of Biochemistry, Faculty of Basic Medical Sciences, College of Medicine, University of Lagos, P.M.B 12003, Lagos State, Nigeria.
O. C. Okafor
Department of Biochemistry, Faculty of Basic Medical Sciences, College of Medicine, University of Lagos, P.M.B 12003, Lagos State, Nigeria.
F. N. Onyekawa
Department of Biochemistry, Faculty of Basic Medical Sciences, College of Medicine, University of Lagos, P.M.B 12003, Lagos State, Nigeria.
*Author to whom correspondence should be addressed.
Abstract
Background: Consolidated bioprocessing (CBP) candidate construction remains gray in the biotechnology of bioethanol production; and recent lead way involved genetic transformation of competent cells with ethanologenic and/or cellulolytic characteristics from natural microorganisms.
Aim: As part of an ongoing study, the ethanologenic property of natural tropical bacteria population isolated from Raphia palm sap was investigated.
Methods: Freshly tapped Raphia palm sap (PW) sample was obtained from a Raphia palm plantation site at Elemu Bus-stop, Jakande, Isolo, Lagos State, Nigeria. The morphological and biochemical characteristics (MBC) as well as 16s rDNA genotyping of the bacteria isolated from PW were used for identification. A representative α–keto acid decarboxylase (pyruvate decarboxylase -PDC) and alcohol dehydrogenase (ADH) productivities of the isolated bacteria in glucose supplemented media were determined by spectrophotometry.
Results: The combination of MBC and 16s rDNA genotyping of the PW bacteria revealed about 6 isolates that were phylogenetically related to Bacillus pumilus, Bacillus subtilis, Paenibacillus validus, Macrococcus spp., Yokenella resenberghei; and Brevibacillus brevis. Meanwhile, efficient PDC productivity (0.5-1.8U/mL) was observed for Bacillus pumilus and Bacillus subtilis at pH 10.0, 25°C, 2.0g substrate concentration, NaNO3 as nitrogen source, and 72 hours incubation period. Bacillus pumilus and Bacillus subtilis efficiently produced ADH (0.5-2.0U/mL) optimally at pH 10.0, 25°C, 1.0g substrate concentration, peptone as nitrogen source, and 96 hours incubation period.
Conclusion: The Raphia palm sap microbiota are efficiently ethanologenic. Thus, their genes can be adapted for genetic transformation in consolidated bioprocessing.
Keywords: Consolidated bioprocessing, bioethanol, genetic transformation, ethanologenic bacteria, 16s rDNA genotyping.