Effect of Gibberellic Acid on Seed Germination and Evaluation of Agrobacterium rhizogenes Strains for the Induction of "Hairy Roots" of Phyllanthus niruri Linn. (Phyllantaceae)
Published: 2020-10-31
Page: 148-162
Issue: 2020 - Volume 3 [Issue 2]
Florent Biduaya Mukeba *
Department of Biology, Faculty of Science, National Pedagogical University, Kinshasa, Democratic Republic of the Congo. and Department of Geostrategy, Geography and Environment, Center of Research of Human Sciences, Kinshasa, Democratic Republic of the Congo. and Department of Biology, Faculty of Sciences, University of Kinshasa, Kinshasa, Democratic Republic of the Congo.
Gédéon Ngiala Bongo
Department of Biology, Faculty of Sciences, University of Kinshasa, Kinshasa, Democratic Republic of the Congo.
Johnny Bopopi Mukoko
Department of Biology, Faculty of Science, National Pedagogical University, Kinshasa, Democratic Republic of the Congo.
Freddy Otono Bulubulu
Department of Biology, Faculty of Science, National Pedagogical University, Kinshasa, Democratic Republic of the Congo. and Laboratory of Vegetal Biotechnology and Molecular Biology, Regional Center of Atomic Energy of Kinshasa, (CREN-K/CGEA), Kinshasa, Democratic Republic of the Congo.
Myriam Mukadi Ngondo
Department of Biology, Faculty of Science, National Pedagogical University, Kinshasa, Democratic Republic of the Congo.
Paulin Kapepula Mutwale
Faculty of Pharmaceutical Sciences, University of Kinshasa, Kinshasa, Democratic Republic of the Congo.
Odette Ngandu Kabena
Department of Biology, Faculty of Sciences, University of Kinshasa, Kinshasa, Democratic Republic of the Congo.
Koto-te-Nyiwa Ngbolua
Department of Basic sciences, Faculty of Medicine, University of Gbado-Lite, Gbado-Lite, Democratic Republic of the Congo. and Department of Biology, Faculty of Sciences, University of Kinshasa, Kinshasa, Democratic Republic of the Congo.
*Author to whom correspondence should be addressed.
Abstract
Phyllanthus niruri L. is a medicinal plant from tropical regions with great pharmacognosic virtues scientifically proven. It is a rare herbaceous wild species in the dry season. Its use for the pharmaceutical industry requires a regular supply and it is independent of climatic hazards. The main aim to find optimal seed storage conditions with reference to the factors that limit the germination of P. niruri seeds and to promote the emergence of dormancy and germination of P. niruri seeds harvested from different agro-ecological sites in Kinshasa. The results of the present study showed high germination rates with GA3, which significantly influenced the germination of the seeds of Phyllanthus niruri. Seeds stored at laboratory temperature (28°C) had a good germination power than those stored at 4°C. In addition, the germination capacity of seeds stored for 7 days seems to be lower than that of those stored for 14 days. Seeds harvested at low altitude (Kisenso site) gave a higher germination rate than those harvested at high altitude (UPN and CREN-K sites). However, the late two sites gave almost the same germination rates.
The Agrobacterium rhizogenes strains A4RSpHKN29, LBA 9402 and ATCC 15834 preserved in the Biotechnology Laboratory of CREN-K did not induce the "Hairy roots" of P. niruri after 27 days of culture on MS medium with sucrose but they conserved their plasmids. Nevertheless, some results obtained showed a low production of vitro-plants and transgenic roots (Hairy roots) from caulinary explants.
Keywords: Phyllanthus niruri L, gibberellic acid, Agrobacterium rhizogenes, plasmids, hairy roots, explants.