Molecular Analysis of Sedative Potential of PREP1 Gene in Mangifera Indica Flower as a Remedy for Insomnia
Published: 2022-07-04
Page: 146-151
Issue: 2022 - Volume 5 [Issue 2]
T. Blessing *
Department of Biotechnology, Nigerian Defense Academy, Kaduna, Nigeria.
A. I. Alhaji
Department of Biotechnology, Nigerian Defense Academy, Kaduna, Nigeria.
G. A. Ajibade
Department of Biological Sciences, Nigerian Defense Academy, Kaduna, Nigeria.
Y. Magaji
Department of Biotechnology, Nigerian Defense Academy, Kaduna, Nigeria.
*Author to whom correspondence should be addressed.
Abstract
Aims: The study was aimed at the molecular characterization of the flower of Mangifera indica for the presence of Pre-sequence Protease 1 gene.
Place and Duration of Study: The fresh flower of Mangifera indica (Mango) were collected using secateures and taken to the Department of Biological Sciences, Nigerian Defense Academy, Kaduna for specific identification and authentication.
Methodology: AccuPrep Genomic RNA Extraction Kit (K-3032) was used for the extraction of the RNA from the flower of Mangifera indica. Molecular weight of the marker gene was used in indicating a high presence of PREP1 gene in Mangifera indica flower.
Results: A high presence of PREP1 gene in Mangifera indica flower was detected using the molecular weight of the amplified bands.
Conclusion: A high presence of PREP1 gene in Mangifera indica flower is a sign of the possibility that the plant sample can feature in the future as a better remedy for treatment of insomnia.
Keywords: Insomnia, sedatives, PREP1 gene, Mangifera indica flower